Das_tool¶
Introduction¶
DAS Tool is an automated method that integrates the results of a flexible number of binning algorithms to calculate an optimized, non-redundant set of bins from a single assembly.
For more information, please check:
BioContainers: https://biocontainers.pro/tools/das_tool
Home page: https://github.com/cmks/DAS_Tool
Versions¶
1.1.6
Commands¶
DAS_Tool
Contigs2Bin_to_Fasta.sh
Fasta_to_Contig2Bin.sh
get_species_taxids.sh
Module¶
You can load the modules by:
module load biocontainers
module load das_tool
Example job¶
Warning
Using #!/bin/sh -l
as shebang in the slurm job script will cause the failure of some biocontainer modules. Please use #!/bin/bash
instead.
To run das_tool on our clusters:
#!/bin/bash
#SBATCH -A myallocation # Allocation name
#SBATCH -t 1:00:00
#SBATCH -N 1
#SBATCH -n 4
#SBATCH --job-name=das_tool
#SBATCH --mail-type=FAIL,BEGIN,END
#SBATCH --error=%x-%J-%u.err
#SBATCH --output=%x-%J-%u.out
module --force purge
ml biocontainers das_tool
DAS_Tool -i sample.human.gut_concoct_contigs2bin.tsv,\
sample.human.gut_maxbin2_contigs2bin.tsv,\
sample.human.gut_metabat_contigs2bin.tsv,\
sample.human.gut_tetraESOM_contigs2bin.tsv \
-l concoct,maxbin,metabat,tetraESOM \
-c sample.human.gut_contigs.fa \
-o DASToolRun2 \
--proteins DASToolRun1_proteins.faa \
--write_bin_evals \
--threads 4 \
--score_threshold 0.6